Series-2 (Jan-Feb 2019)Jan-Feb 2019 Issue Statistics
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| Paper Type | : | Research Paper |
| Title | : | Restriction of Enzyme In EEL MT DNA |
| Country | : | India |
| Authors | : | Edwin Jefri || Reni Mulyani || Novita || Neneng Nurbaeti |
 |
: | 10.9790/264X-0501020103  |
Abstract: his study was conducted to determine the genetic diversity of eel from the estuary of the Cimandiri Palabuhanratu River. Research on the genetic diversity of eels is very important for maintaining genetic resources, species and eel fish ecosystems. Genetic resources are the basic stages in the effort to protect eel resources in Indonesia. From the results of the study, eel fish taken from the mouth of the Cimandiri River, Pelabuhan Ratu is from the genus Anguilla. The samples are then preserved with absolute ethanol solution (96%), followed by the extraction process, amplification of PCR (Polymerase Chain Reaction), electrophoresis, and limitation of DNA sequences by enzymes HaeIII, Hin6I, RsaI, TaqI and NdeII). From the results of PCR analysis that Sidat fish in the Cimandiri.......
Keywords: eel, larvae, juvenils, Palabuhanratu, morphological, enzymes, restriction.
[1]. Affandi R. 2005. Strategy for Utilizing Sidat Fish Resources, Anguilla spp. in Indonesia. Indonesian Psychology Journal, Volume 5, Number 2
[2]. Affandi R, Budiardi T, Wahju RI, Taurusman AA. 2013. Maintenance of eel fish with a circulating water system. Journal of Indonesian Agricultural Sciences (JIPI), April 2013 Vol. 18 (1): 55-60
[3]. Aoyama J, Wouthuyzen S, Miller MJ, Minegishi Y, Kuroki M, Suharti SR, Kawakami T, Sumardiharga KO, Tsukamoto K. 2007: Distribution of leptocephali of the freshwater eels, genus Anguilla, in the waters off west Sumatra in the Indian Ocean . Env. Biol. Fish. 80: 445–452.
[4]. Aoyama J. 2009: Life history and evolution of migration in catadromous eels (genus
[5]. Anguilla). Aqua-BioSci. Monogr. 2: 1–42..
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| Paper Type | : | Research Paper |
| Title | : | Nanotechnology: Applications in Medicine and Drug Delivery- A Review |
| Country | : | India |
| Authors | : | S. Janardana Reddy || B. Kiran Kumar |
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: | 10.9790/264X-0501020420 |
Abstract: The use of nanotechnology in medicine and more specifically drug delivery is set to spread rapidly. Currently many substances are under investigation for drug delivery and more specifically for cancer therapy. Interestingly pharmaceutical sciences are using nanoparticles to reduce toxicity and side effects of drugs and up to recently did not realize that carrier systems themselves may impose risks to the patient. Nano medicine is a comparatively new field of science and technology. Brief explanation of various types of pharmaceutical nano systems is discussed. Classification of nano materials based on their dimensions is also explained. Applications of Nanotechnology in diverse fields such as health and medicine, electronics, energy and environment, are discussed. Applications of various.........
Key Words: Nano devices; nano tubes, dendrimers, nano crystals, Nano material; Nano medicine; Nano pharmaceutics; Drug delivery.
[1]. Martin CR. Welcome to nanomedicine. Nanomedicine. 2006;1(1):5.
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[3]. Fenyman R. "There‟s Plenty of Room at the Bottom." Science 1991, 254: 1300-1301.
[4]. Taniguchi N. 1974. "On the Basic Concept of "Nano-Technology‟" Proc. Intl. Conf. Prod. Eng. Tokyo, Part II, Japan Society of Precision Engineering.
[5]. Murray CB, Kagan CR, Bawendi MG: Synthesis and characterisation of monodisperse nanocrystals and close-packed nanocrystal assemblies. Annu Rev Mater Sci 2000, 30:545-610.
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| Paper Type | : | Research Paper |
| Title | : | In Vitro Direct Propagation and Genetic Stability of Sugarcanecultivar (G.2003/49) |
| Country | : | Egypt |
| Authors | : | H.A.M. Abd-Alla || A.B. Abdel-Razik || H.S. Taha || Z.A. Ali || S.A. Ibrahim |
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: | 10.9790/264X-0501022128 |
Abstract: Micropropagation of sugarcane cultivar(G.2003/49) through using shoot tips meristem consider and successfully protocol to obtained uniformed diseases- free plants. The obtained results revealed that peak the maximum number of shootlets, leaves and nodes were recorded on MS medium supplement with 2mg/l KIN + 0.25 mg/l NAA. However, the highest shootlets (18.6 cm) wasreported on MS mediumsupplemented with 0.25 mg/l NAA. On the other hand, roots induction indicated that the highest root length (2 cm) resulted from ½MS contained 2mg/l IAA+ 1 mg/l NAA. Whereas, the maximum roots number (7.3) was recorded on ½MS fortifiedwith 1mg/l NAA. Furthermore, stability or somaclonal variation not obtained through genetic analysis of mother leaf tissue (as a control) compared 6 subcultures of in vitro resulted plantlets summarized that all obtained bands were monomorphic..
Key Words: Micropropagation, shoot tip meristem, sugarcane, kin, NAA, IAA. Abbreviations: KIN, Kinetin; NAA, Naphthalene acetic acid; IAA, indole-3-acetic acid; MS, Murashige and Skoog..
[1]. Jahangir, G.Z., and Nasir, I.A. (2010). Various hormonal supplementations activate sugarcane regeneration invitro. 2(4): 231-237.
[2]. Jalaja, N.C., D. Neelamathi, and T.V., Sreenivasan(2008). Micropropagation for quality seed production in sugarcane in Asia and the Pacific.
[3]. FAOSTAT . 2014, food and agriculture organization of the United Nations statistics, available at http:// faostat.fao.org/site/362/.
[4]. Senger,K., Sengar, R.S. and Garg .S.K. (2010). Daveloping an efficient protocol through tissue culture techniques for sugarcane micropropagation.Bioinfo bank library acat, 18:56.
[5]. Snyman S. J., Meyer, G. M., Richards, J. M, Haricharan. N, Ramgareeb. S, Huckett, B. I.(2006). Refining the application of direct embryogenesis in sugarcane: effect of the developmental phase of leaf disc explants and the timing of DNA transfer on transformation efficiency. Plant Cell Rep 25:1016-1023.
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| Paper Type | : | Research Paper |
| Title | : | Efficiency of Taraxacumofficinale(Dandelion) leaf Extract in alleviating Ulcer Occasioned by long ingestion of Non-Steroidal Anti-inflammatory Drug (NSAID) in Wister Rats. |
| Country | : | Nigeria |
| Authors | : | Berezi, E.P. || Uwakwe, A.A. || Monago-Ighorodje, C.C. || Nwauche, K.T. || Soro, A.E. || Mirinn, E |
|
: | 10.9790/264X-0501022936 |
Abstract: The main objective of this study is to investigate the potentials of aqueous extract of Taraxacumofficinaleleaf on injury occasioned by thelong ingestion of Non-Steroidal Anti-inflammatory Drug (NSAID) Ibuprofen, in wistar rats. Rats weighing between 108 – 200g were used for the study. The ulcer was confirmed by administering 20mg/kgbw of the NSAID for 3 days. The rats were randomly divided into five (5) groups. Group 1 rat received distilled water that served as the normal control; group 2 animal had no treatment (Ulcer Control), group 3 received 20mg/kgbw Omeprazole (Reference drug), while group 4 and 5 rats were treated with 250mg/kgbw and 500mg/kgbw of plant extract administered.......
Key Words: Taraxacumofficinale, Ulcer, Non-Steroidal Anti-Inflammatory Drug, gastric Mucosa, ulcer
[1]. Abdulla, M.A., Ahmed, K.A.A., Al-Bayaty, F.H. and Masood, Y. (2010). Gastroprotective effect of Phyllanthusniruri leaf extract against ethanol-induced gastric mucosal injury in rats. African Journal of Pharmacy and Pharmacology.4(5): 226 – 230.
[2]. Aguwa, CN. andNwako, S.O. (1988). Preliminary studies on the Root Extract of Nauclealati folia Smith for Anti-Ulcer Properties. Nigeria Journal of Pharmaceutical Sciences. 4(1): 16 – 23.
[3]. Akhtar, A.H. and Ahmed, K.U. (1995). Anti-Ulcerogenic evaluation of the methanolic extract of some indigenous medical plants of Pakistan in Aspirin ulcerated rats. Journal of Ethnopharmacology. 46:1-6.
[4]. Al Rashdi, A.S.,Salama, S.M.,Alkiyumi, S.S., Abdulla, M.A., Hadi A. Hamid, A.,Abdelwahab, S.I.,Taha, M.M.,Hussani, J. and Asykin, N. (2012). Mechanism of Gastroprotective Effects of Ethanolic Leaf Extract of JasminumSambac against HU/ Ethanol – Induced Gastric Mucosal Injury in Rats. Evidence Based Complementary and Alternative Medicine. Article786426: 1 – 15.
[5]. Amin Mir, M., Sarohney, S.S. andJassal, M.M.S. (2013). Qualitative and quantitative analysis of phytochemicals of TaraxacumOfficinale. Woodpecker Journal of Pharmacy and pharmacology.2(1): 001 – 005.
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| Paper Type | : | Research Paper |
| Title | : | The Effect of Ginger Extract (Zingiber officinale Roscoe) on antioxidant enzymes and free radical in rabbits |
| Country | : | Libya |
| Authors | : | Ahlam A.K. Al-Ailla || Fayrouz A. Khaled |
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: | 10.9790/264X-0501023740 |
Abstract: Ginger rhizome has a long history of use in traditional systems of medicine has been found to possess antioxidant effect that can control the generation of free radicals. Free radical level has been reported to be high in cancer cells. The aim of this study was to observe the effect of ginger extract on antioxidant status in rabbits. The effects of ginger on plasma glutathione (GSH), glutathione peroxidase (GPx), glutathione S- transferase (GST), catalase (CAT), superoxide dismutase (SOD) activities and thiobarbituric acid-reactive substances (TBARS) during the 12-week. Treatment with ginger caused significant (P<0.05) increase in the activity of GSH, GPx, GST, SOD and CAT in plasma compared to control, while (TBARS) was significantly (P<0.05) decreased compared with control group.
Key Words: antioxidant enzymes, free radical and Zingiber officinale Roscoe
[1]. Oke, J. M. and Hamburger, M. O. (2002). Screening of some Nigerian Medicinal Plants for antioxidant activity using 2,2, diphenyl-picryl-hydrazyl radical. Afr. J. Bio. Res., 5: 77-79.
[2]. Chinedu, P. A., Ijeoma, E., Olushola, A. and Ayobami, O. A. (2011). Polyphenolic a content and antioxidant activity of Hibiscus sapdariffa calyx. Res. J. Med. Plant., 5: 557-566.
[3]. Sofidiya, M. O., Odukoya, O. A., Familoni, O. B. and Inya-Agha, S. I. (2006). Free Radical Scavenging Activity of Some Nigerian Medicinal Plant Extracts. Pakistan. J. Biol. Sci., 9: 1438-1441.
[4]. Haksar, A., Sharma, A., Chawla, R., Kumar, R., Arora, R. and Singh, S. (2006). Zingiber officinale exhibits behavioral radioprotection against radiation. Pharm. Biochem. Behaviour., 84: 179-188.
[5]. Morakinyo, A. O., Achema, P. U. and Adegoke, O. A. (2010). Effect of Zingiber officinale (Ginger) on sodium arsenite-induced reproductive toxicity in male rats. African. J. Bio. Med. Research., 13: 39-45
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| Paper Type | : | Research Paper |
| Title | : | Hypocholesterolemic Efficacy of Annosa Squamosa (L.) Extract In Mice Diabetic Models |
| Country | : | India |
| Authors | : | Sanjay Kumar Sharma || Moti Lal Gupta || Baidyanath Kumar |
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: | 10.9790/264X-0501024145 |
Abstract: The majority of Type-2 DM patients suffer from visceral obesity and they often have high circulating levels of lipids including cholesterol, triglycerides, low levels of high density cholesterol, which also contribute to the development of vascular complications. In the present study the efficacy of methanol extract of leaves of Annona squamosa on serum lipid profile of diabetic mice was studied. The results clearly indicated that Diabetic mice treated with A. squamosa extract (DT150) showed significantly lower values of serum TC (-26.5%; p<0.001) and TGs (-55.6%; p<0.001), when compared with the DC counterparts. The DT250 treatment showed superior lowering effects compared with the DC counterparts as well as DT150 group mice by (-34.7%; p<0.001) on serum TC levels and (-50%; p<0.001) on TGs levels. Contrarily, treatment with rosiglitazone (DTRGZ) showed (-33.2%; p<0.001) on TC levels and (-09.6%; p<0.001) on TGs levels compared with diabetic control mice..
Key Words: Annona squamosa, Lipid profile, Streptozotocin, Mice
[1]. Basnet. P, Kadota. S, Shimizu, M (1994): The hypoglycemic activity of Swertia japonica extract in streptozotocin induced hyperglycemic rats. Phytothr res, 8: 55-57
[2]. Saxena. A. M., Bajpai. M B., Murthy. P. S, Mukherjee. S. K (1993): Mechanism of blood sugar lowering by a swechirin-containing hexane fraction (SWI) of Swertia chirayita. Indian J Exp Biol, 31: 178-181
[3]. Sellamuthu. P. S, Muniappan. B P, Perumal. S M, Kandasamy. M (2009): Antihyperglycemic effect of mangiferin in streptozotocin induced diabetic rats. J. Health. Sci, 55: 206-2014
[4]. Vaidya H, Rajani M, Sudarshan V, Padh H, Goyal R, Swertiamarin (2009): A lead from Enicostemma littorale Blume. For anti-hyperlipidemic effect. Eur J. Pharm, 617: 108-112
[5]. Garcia, M. J., McNamara, P. M., Gordon, T. and Kannel, W. B. Diabetes, 1974, 23, 105-112.American Diabetes Assn., Diab. Care, 1995, 18, 1510-1518.
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| Paper Type | : | Research Paper |
| Title | : | Biology and Growth Characteristics of Edible Mushroom: Agaricus compestris, Agaricus bisporous, Coprinus comatus |
| Country | : | India |
| Authors | : | Kameshwar Singh || Gajendra Prasad |
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: | 10.9790/264X-0501024659 |
Abstract: Agarics, commonly known as gill- fungi, mushroom or toadstools produce conspicuous basidiocarps. Agarics may be edible, poisonous or unpalatable. Edible agarics are commonly known as mushrooms and poisonous ones the "toad stools". They are mostly saprophytic, growing commonly in lawns, pasture and gardens. In the present study the biology and growth characteristics of three edible mushrooms viz. Agaricus bisporus, A. compestris and Coprinus comatus was studied. Among different carbon sources sucrose, fructose, maltose, lactose and glucose favoured maximum mycelia growth rate. Soybean meal, bran and yeast extract were found to be the best nitrogen...........
Key Words: Mycelial growth, Agarics, Carbon sources, Nitrogen sources, Growth factors, Vitamins
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[2]. Koyyalamusi, SR, Jeong S C, Song, C.H, K.Y, Pang, G (2009): Vitamin D2 formation and bioavailability from Agaricus bisporus button mushrooms treated with ultraviolet irradiation. Jo urnal of Agriculture and food Chemistry 57 (8): 3351-5.
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[4]. Sieger, A.A. (2010): Bulletin of the Puget sound Mycological Society. http://www.psms.Org/sporeprints/sp 338. html.
[5]. Ammirati, JF, Traquair, JA, Horgen PA(1985). Poisonous mushrooms of Canada: Including others in edible Fungi: Markham, Ontario: Fitzhenry & Whiteside in cooperation with Agriculture Canada and the Canadian Government publishing centre, Canada..
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| Paper Type | : | Research Paper |
| Title | : | Studies on Inhibitory Effect of Phyto Extracts over Growth and Exotoxin Activity of Pseudomonas Aeruginosa Strain Pp76 |
| Country | : | India |
| Authors | : | Shivashankar. M |
|
: | 10.9790/264X-0501026069 |
Abstract: Pseudomonas aeruginosa is a common bacterium that can cause disease in animals and humans. The source of pathogenicity of this bacterium is a potent Pseudomonas exotoxin (PE). P. aeruginosa is naturally resistant to a large range of antibiotics. The objective of this present work project is to control Pseudomonas infection through therapeutic methods based on different medicinal plant extracts of turmeric, ginger, clove oil from clove, Euphorbia hirta, Fenugreek and Alangium. The main aim of this study is to inhibit exotoxin activity by using different phytotherapuetics. Exotoxin was extracted from Pseudomonas and to be purified. The domain regions of Exotoxin A, Exotoxin S and Exotoxin Y have to be analyzed and modeled by using Bioinformatics techniques. Docking of different plant derivatives with Exotoxins were done to determine the best phytotherapeutics to control toxin activity..
Key Words: Pseudomonas origins, Exotoxin A, Exotoxin S, Exotoxin Y, Phytotherapeutics, Bioinformatics, docking.
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[5]. Alonso-Paz E, Cerdeiras M.P., Fernandez J., Ferreira F., Moyna P., Soubes M., Vazquez A., Veros S. and Zunno L., Screening of Uruguayan medicinal plants for antimicrobial activity. J. Ethanopharm. 45: 67-70 (1995).
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| Paper Type | : | Research Paper |
| Title | : | Heterosis and Heritability Estimates of Amino Acids and Carbohydrates in the Theobroma Cacao/Phythophthora megakaryaInteraction |
| Country | : | |
| Authors | : | Pierre François Djocgoue || Pierre Effa Onomo || Martine Louise Ondobo || Jules Christian Djoko Kouam || Annice Helene Mewouo || Jude Manga Ndjaga || Nicolas Niemenak |
|
: | 10.9790/264X-0501027080 |
Abstract: Phythophthoramegakarya is the principal agent of the black pod disease of cocoa (Theobroma cacao L.) in Cameroon and is responsible for 80% loss of cocoa production. The use of hybrid clones displaying resistance to diseases is considered as one of the principal methods of ameliorating crop losses.In order to assess the T. cacao/P. megakarya interaction, comparative analysis of amino acids and sugars were conducted on healthy, wounded and infected leaves of SCA12, ICS40, SNK16 and T79/501 clones and their hybrids (families F12, F40, F79 and F16) derived from reciprocal crossings. Injury and infection were characterized by an increase in amino acids and a decrease in soluble sugars in F12-F40 families. Analysis of free amino acids revealed the absence of phenylalanine. ICS40.........
Key Words: Black pod disease, cocoa clones, GABA, hybrid vigor,reciprocal crossings.
[1]. Iwaro, A.D., Sreenivasan, T.N., Umahara, P. 1997. Phytophthora resistance in cacao (Theobroma cacao): Influence of pod morphologicalcharacteristics. Plant Pathology 46, 557-565.
[2]. Nyassé, S. 1997. Étude de la diversité de Phytophthora megakarya et caractérisation de la résistance du cacaoyer (TheobromacacaoL.) à cepathogène. Thèse de doctorat, Institut National Polytechnique de Toulouse, France.
[3]. Ndoumbe-Nkeng, M., Efombagn, M. I. B., Nyasse, S., Nyemb, E., Sache, I., Cilas, C. 2009. Relationships between cocoa Phytophthora podrot disease and climatic variables in Cameroon. Canadian Journal of Plant Pathology 31(3), 309-320.
[4]. Despréaux, D., Cambrony, D., Clément, D. Partiot, M., 1988. Étude de la pourriture brune des cabosses du cacaoyer au Cameroun: définition de nouvelles méthodes de lutte. In: 10th Proceeding of International Cocoa Research Conference, Santo Domingo, DominicanRepublic, 407-412.
[5]. Berry, D. and Cilas, C. 1994. Etude génétique de la réaction à la pourriture brune des cabosses chez des cacaoyers issus d'un plan decroisements diallèle. Agronomie 14, 599-609..
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| Paper Type | : | Research Paper |
| Title | : | In vitro evaluation of phytochemical and antibacterial activity of wild species of Solanum L. |
| Country | : | India |
| Authors | : | Pooja Singh || Prashant Singh || Anjana Singh || M. P. Singh |
|
: | 10.9790/264X-0501028187 |
Abstract: The potential antimicrobial activity of medicinal plants need identification and screening due to increasing use of synthetic drugs, side effects and antibiotic resistance of pathogenic microorganisms. In-vitro, antimicrobial activity of methanolic extracts of Solanumnigrum, S.villosum, S.torvum, S.surratense, S.sysimbrifolium, S.diphyllum and S.incanumagainst two Gram positive (Staphylococcus aureus and Bacillus subtilis) and three Gram negative bacteria(E.coli, Salmonella typhi and Proteus vulgaris) have been screened and evaluated phytochemically in this study. The zone of inhibition was determined by agar disc diffusion method varied with plant..............
Key Words: Wild Solanum,Phytochemical screening, Antimicrobial activity, Inhibition zone
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